We use the Mo-Bio DNA isolation kit to isolate our DNA.


DNA isolation is the process of collecting and isolating the DNA for analysis and PCR.


Isolation has four basic steps:
  1. Cell Lysis
  2. Removing Membrane
  3. Removing Proteins
  4. Precipitating the DNA

Cell lysis is the process of breaking down the cell to make the DNA accessible and expose it. Membrane lipids are fats and oils on the cell membrane that need to be removed in order to access the DNA. Precipitating DNA is the process of using ethanol to purify and concentrate DNA.


During DNA Isolation, it is very important to avoid contaminating the sample. If the sample is contaminated, one could isolate the wrong DNA.


To avoid contamination, we:
  • Wear gloves
  • Avoid breathing on the samples
  • Tie back hair
  • Sterilize equipment


The next step of our process is PCR.

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