The biosub is originally developed from the biocoil, a photosynthetic bioreactor, BioCoil. The Sewage Sisters, whose goal was to clean the sewage from our lake, first built the biocoil in 1993. At that time the group measured the amounts of nitrates and phosphates being used by the algae. The project has continued for more than a decade and has slowly developed and evolved from the original idea. A new challenge presented itself when Lloyd Godson, BioSub, contacted our class and asked if we could do the same system, but measure the amount of carbon dioxide being used and the amount of oxygen being released. In the spring of 2006 three members joined the original four members to help contribute ideas to the group. As new designs and ideas were created and as time went these designs developed and improved to make a system that would fit Lloyd’s.